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. 2021 Oct 20;10(11):2240. doi: 10.3390/plants10112240

Table 2.

Validation parameters for the ultra-performance liquid chromatography (UPLC) method.

Flavonoid/Anthocyanin Component Linearity (r2) Slope (y) Response (Sy) Sy/y LOD *
(µg·mL−1)
LOQ **
(µg·mL−1)
Rutin 0.999303 0.2737 5.2262 19.0921 63.00 190.92
Luteolin 0.999692 0.2745 4.9727 18.1111 59.76 181.11
Quercetin 0.999667 0.2756 4.6358 16.8164 55.49 168.16
Cyanidin-3-O-glucoside chloride 0.998590 0.2767 4.3319 15.6526 51.65 156.52
Peonidin-3-O-glucoside 0.999506 0.2757 4.6096 16.7147 55.15 167.14
Pelargonidin-3-O-glucoside 0.998351 0.2754 4.7720 17.3254 57.17 173.25

* Limit of detection; ** Limit of quantification.