Figure 2.
(a) SPR characterization of 2.26 μM amelotin on a NTA chip, with NA protein as analyte with a concentration of 2.26 (BMax 129.56°), 1.13 (BMax 415.89°) and, 0.56 μM (BMax 645.37°). The dissociation constant was calculated to be 1.25 × 10−7 ± 4.34 × 10−9, 1.61 × 10−7 ± 2.43 × 10−9 and 1.75 × 10−7 ± 8.29 × 10−9, respectively, as described in the Supplementary Materials. (b) FTIR examination of NA protein, amelotin, co-assembly and synthetic hydroxyapatite crystals. The main mineral observed in the co-assembly samples was hydroxyapatite.