TABLE 1.
Multilocus genotypes of C. immitis isolatesa
| Locus | Occurrence of polymorphism in isolated:
|
Site (base)b | Enzymec | ||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 2002B | 2004B | 2005B | 2006B | 2007B | 2008B | 2009B | 2010B | 2011B | 2012B | 2013B | 2014B | 2015B | 2017B | 2649B | 2267B | 2268B | 2269B | 2270B | 2271B | 2273B | 2274B | 2275B | 2276B | 2277B | 2278B | 2279B | 2280B | 2281B | 3255B | 3257B | 3258B | 3262B | 3264B | 2808B | 2610B | 435B | 1036 | 3272 | |||
| SNP | |||||||||||||||||||||||||||||||||||||||||
| VL | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 70 (t/c) | HinfI |
| CTS1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1441 (g/c) | MnlI |
| OR | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 69 (a/g) | MaeIII |
| ITS | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 461 (t/c) | HaeIII |
| VK | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 290 (g/c) | MnlI |
| BL | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 215 (–/a) | HinfI |
| CAG | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 377 (t/g) | HaeIII |
| CNS | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 64 (c/t) | MspI |
| HSP | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 252 (t/c) | EcoRV |
| BOR | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 135 (t/c) | TaqI |
| BGL2 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 253 (t/c) | SSCP |
| RAN1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 192 (t/c) | NruI |
| 621 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | — | 1 | 1 | 1 | 1 | 1 | 1 | 255 (g/a) | Sau96I |
| SP | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 425 (g/c) | MnlI |
| STR | |||||||||||||||||||||||||||||||||||||||||
| 621r | 3 | 1 | 8 | 4 | 3 | 4 | 2 | 3 | 4 | 5 | 5 | 8 | 4 | 7 | 5 | 8 | 4 | 4 | 2 | 4 | 7 | 3 | 6 | 4 | 2 | 2 | 3 | 5 | 7 | 7 | 3 | 7 | 7 | 2 | 3 | 7 | 4 | 0 | 0 | 121 | SV |
| B34 | 2 | 2 | 6 | 2 | 2 | 3 | 5 | 3 | 3 | 1 | 3 | 3 | 5 | 5 | 3 | 6 | 2 | 3 | 3 | 2 | 3 | 3 | 2 | 3 | 2 | 3 | 6 | 3 | 3 | 2 | 3 | 2 | 3 | 3 | 3 | 2 | 1 | 1 | 1 | 100 | SSCP |
All isolates came from Bakersfield, except 1036 and 3272, which were from patients who gained their infections in Arizona and Guatemala, respectively. These are representatives of the non-California species of C. immitis. SNP loci are loci containing single-nucleotide polymorphisms; STR loci are loci containing short tandem repeats.
Position of the polymorphic nucleotide in GenBank sequence. Allele 0/allele 1 nucleotides are shown. For loci 621r and B34, the numbers of repeats found in the STR sequence are shown.
Enzymes used to score the alleles are shown. BGL2, B34, and 621r were genotyped by SSCP or size variation (SV).
Reported as presence (1) or absence (0) for SNP loci or as number of repeats for STR loci. —, no data were obtained.