Table 3.

Obtained downstream targets of the EPAS1 from the literature.

Target Gene		Species		Validation		Reference
Name (Synonim)	Entrez ID	Name	ID	Experimental Methods	Cell Line and/or Tissues
VEGFA (VEGF)	7422	Human	9606	siRNA HIF1A/EPAS1	Renal carcinoma RCC4 and 786-O (no VHL function)	[54]
VEGFA (VEGF)	7422	Human	9606	DNA microarray and northern blot	Renal carcinoma 786-O (no HIF1A) and HEK293 TET-on cells	[55]
VEGFA (VEGF)	7422	Human	9606	siRNA HIF1A/EPAS1, ELISA	Renal carcinoma RCC4 and SKRC28	[56]
SLC2A1 (GLUT1)	6513	Human	9606	siRNA HIF1A/EPAS1	Renal carcinoma RCC4 and 786-O (no VHL function)	[54]
SLC2A1 (GLUT1)	6513	Human	9606	Overexpression of HIF1A/EPAS1, immunoblotting	Renal carcinoma 786-O, RCC4 and SKRC28	[56]
PLAUR (uPAR)	5329	Human	9606	siRNA HIF1A/EPAS1	Renal carcinoma RCC4 and 786-O (no VHL function)	[54]
SERPINE1 (PAI-1)	5054	Human	9606	siRNA HIF1A/EPAS1	Renal carcinoma RCC4 and 786-O (no VHL function)	[54]
CCND1 (cyclin D1)	595	Human	9606	siRNA HIF1A/EPAS2, overexpression of HIF1A/EPAS1, immunoblotting	Renal carcinoma 786-O	[56]
BNIP3	664	Human	9606	Overexpression of HIF1A/EPAS1, immunoblotting	Renal carcinoma 786-O, RCC4 and SKRC28	[56]
TGFA	7039	Human	9606	siRNA HIF1A/EPAS1, ELISA	Renal carcinoma RCC4 and SKRC28	[56]
PLIN2 (ADRP)	123	Human	9606	DNA microarray and northern blot	Renal carcinoma 786-O (no HIF1A) and HEK293 TET-on cells	[55]
NDRG1	10397	Human	9606	DNA microarray and northern blot	Renal carcinoma 786-O (no HIF1A) and HEK293 TET-on cells	[55]
ADM	133	Human	9606	DNA microarray and northern blot	Renal carcinoma 786-O (no HIF1A) and HEK293 TET-on cells	[55]
DNMT1	1786	Human	9606	ChIP-qPCR, Luciferase assay	Lung cancer cell lines, HEK293 cells	[37]
EPO	3415	Mouse Human	10090 9606	Cre-mediated inactivation of HIF1A/EPAS1 in mice and siRNA in cell lines, RT-PCR, ChIP	Mice liver tissue and liver Hep3B cells	[57]
EPO	3415	Human	9606	siRNA HIF1A/EPAS1, immunoblotting, RNase protection assay	Liver Hep3B cells and neuroblastoma Kelly cells	[58]