Table 1.
Specifications of primers used in RT-qPCR.
| Gene | Size (bp) | Sequence 5′–3′ | MT | NCBI Reference Sequence |
|---|---|---|---|---|
| Il-1β | 93 | FW: AGGCTTCCTTGTGCAAGTGT | 60 °C | NM_031512.2 |
| RV: AGGTCATTCTCCTCACTGTCG | ||||
| Il-6 | 92 | FW: TCATTCTGTCTCGAGCCCAC | 60 °C | NM_012589.2 |
| RV: CTCCGCAAGAGACTTCCAGC | ||||
| Il-10 | 95 | FW: GACGCTGTCATCGATTTCTCC | 60 °C | NM_012854.2 |
| RV: GCTCCAAGACAAAGGTGTCTAC | ||||
| Tnf-α | 100 | FW: ATGGGCTCCCTCTCATCAGT | 60 °C | NM_012675.3 |
| RV: TGGTTTGCTACGACGTGGG | ||||
| Ki-67 | 100 | FW: GGGTTTCCAGACACCAGACC | 60 °C | NM_001271366.1 |
| RV: CCAGGAAGACCAGTTAGAACC | ||||
| Ef-1α | 91 | FW: CTTTGGACTGCATTCTGCCG | 60 °C | NM_175838.1 |
| RV: GTGCCAATGCCGCCAATTTT |
Bp: base pairs, MT: melting temperature, FW: forward, RV: reverse.