Table 2.
Cell Line | Tissue Origin and Morphology | References | CNF1 Described Effects | CNF1 Preparation |
---|---|---|---|---|
HMEC-1 (Human Microvascular Endothelial Cell line-1) |
hu dermal endothelium, immortalized (SV40 T-antigen), endothelial-like | [67] |
|
recombinant, GST fusion protein |
HEK 293 (Human Embryonic Kidney 293) |
hu embryonic kidney, transformed, tumorigenic, epithelial | [11] * [9] ‡ |
|
recombinant, * purified by chromatography ‡ GST fusion protein |
HEK 293T (Human Embryonic Kidney 293T) |
hu embryonic kidney, transformed, HEK 293 derivative expressing SV40 T-antigen, epithelial | [68] |
|
recombinant, His-tagged protein |
HBMEC-60 (Human Bone Marrow Endothelial Cell line-60) |
hu bone marrow, immortalized (HPV16 E6/E7), endothelial | [69] |
|
recombinant, purified by chromatography |
HBEC-5i (Human Brain Endothelial Cell line-5i) |
hu brain cerebral cortex, immortalized (SV40 T-antigen), endothelial | [22,69] |
|
recombinant, purified by chromatography |
SV-HUC-1 | hu ureter, immortalized (SV40 T-antigen), epithelial | [27] | multinucleation | recombinant, His-tagged protein |
MesEnd (Mesenteric Endothelial) |
mouse mesenteric microvascular, immortalized (SV40 T-antigen), | [70] |
|
recombinant, GST fusion protein |
MyEnd (Myocardial Endothelial) |
mouse microvascular myocardial, immortalized (SV40 T-antigen) | [70,71,72] |
|
recombinant, GST fusion protein |
HaCaT | hu skin, spontaneously immortalized, keratinocyte | [73] |
|
not specified |
NIH 3T3 | mouse embryonic, spontaneously immortalized, fibroblasts | [6,33,37,74] |
|
recombinant, GST fusion protein |
3T3-Swiss albino | mouse embryo, spontaneously immortalized, fibroblasts | [53] |
|
recombinant, His-tagged protein |
3T3-L1 | mouse embryo, substrain of 3T3-Swiss albino, preadipocytes fibroblasts |
[75] |
|
recombinant, His-tagged protein |
C2C12 | mouse muscle, spontaneously immortalized, myoblast | [76] |
|
recombinant, purified by chromatography |
Vero | monkey kidney, spontaneously immortalized, epithelial | [5,11,56] |
|
recombinant, purified by chromatography |
MDCK (Madin-Darby Canine Kidney) |
dog kidney, spontaneously immortalized, epithelial | [77] |
|
recombinant, GST fusion protein |
PAE (p23 clone) (Porcine Aorta-derived Endothelial) |
pig aorta | [78] |
|
recombinant, GST fusion protein |
* purified by chromatography; ‡ GST fusion protein