Table 1.

Summary of various standard methods used for dietary fiber determination.

AOAC Method	Measurement Type	Enzymes Used	Other Properties
985.29	Total HMWDF	Bacterial α-amylase, protease, and amyloglucosidase	1 g sample, phosphate buffer
991.42	IDF in food products	Bacterial α-amylase, protease, and amyloglucosidase	1 g sample, phosphate buffer
991.43	IDF and SDFP	Bacterial α-amylase, protease, and amyloglucosidase	1 g sample, Tris or MES buffer, pH = 8.2
993.19	SDFP in food products	Bacterial α-amylase, protease, and amyloglucosidase	1 g sample, phosphate buffer
993.21	HMWDF (samples with more than 10% fiber and less than 2% starch)	Carried out without enzymes	0.1 g sample, without buffer,
994.13	HMWDF	Bacterial α-amylase and amyloglucosidase	0.25–0.5 g sample, acetate buffer, also provides composition of sugars and Klason lignin
2001.03	HMWDF and SDFS	Bacterial α-amylase, protease, and amyloglucosidase	1 g sample, phosphate buffer, only for samples free of resistant starch
2009.01	HMWDF and SDFS	Pancreatic α-amylase, protease, and amyloglucosidase	1 g sample, meleate buffer, pH = 6.0, available for any kind of food
2011.25	IDF, SDFP, and SDFS	Pancreatic α-amylase, protease, and amyloglucosidase	1 g sample, meleate buffer, pH = 6.0, available for any kind of food

HMWDF = higher-molecular-weight dietary fiber; IDF = insoluble dietary fiber; SDFP = dietary fiber that dissolves in water but not in 76% aqueous ethanol; SDFS = dietary fiber that is soluble in both water and in 76% aqueous ethanol. Total dietary fiber = HMWDF + SDFS; HMWDF = IDF + SDFP.