Figure 2.

Transfection of the CLU gene promoter in human corneal epithelial cells. (A) Representation of the CLU/CAT recombinant plasmids used for transfection analyses. Numbers indicate the 5′ position relative to the CLU theoretical mRNA start site (indicated by a curved arrow). (B) CAT activities measured following transfection of the CLU/CAT constructs shown in panel (A) in human corneal epithelial cells. CAT activity is expressed relative to the level directed by CLU/-203 construct. Values considered to be statistically significant from those obtained with the CLU/-203 construct are indicated (* and ****: p values < 0.05 and < 0.0001, respectively). ns: Values considered to be statistically non-significant. (C) Schematic representation of the basal promoter (basal P) and both the proximal and distal silencers along the CLU promoter and 5′-flanking sequence, based on the transfection results from the panel (B).