TABLE 3.
Examination of 75 sputum samples with various oligonucleotide probes by FISH and by conventional microbial culture
| Procedure and result | No. of samples containing the following species (probesa):
|
|||||
|---|---|---|---|---|---|---|
| P. aeruginosa (PsaerA-PsaerB) | S. aureus (Staaur) | H. influenzae (Haeinf) | B. cepacia (Burcep) | Burkholderia spp. (Burkho) | S. maltophilia (Stemal) | |
| Culture | ||||||
| Positive | 59 | 14 | 3 | 6 | 6 | 6 |
| Negative | 16 | 61 | 72 | 54 | 54 | 54 |
| Culture positive | ||||||
| FISH positive | 55 | 10 | 3 | 6 | 6 | 5 |
| FISH negative | 4 | 4 | 1 | |||
| Culture negative | ||||||
| FISH positive | 4 | 1 | ||||
| FISH negative | 12 | 25b | 24b | 25b | 25b | 25b |
a
Oligonucleotide probes used for FISH.
b
Only 25 culture-negative sputum samples were examined by FISH with the respective FISH probes.