Figure 5.

Amino acid utilization profile of K. rhaeticus ENS9b cells grown in M9 medium individually containing 22 L-amino acids. The test was conducted in 96-well microtiter plate wells containing a total volume of 200 µL/well. The experimental controls included M9 medium containing cells but devoid of amino acid supplementation (M9+ino control) and M9 medium devoid of cells and amino acids (M9 control). The microtiter plates were incubated at 30 °C for 8 days and the OD_600nm measurements were taken once every 24 h. The presented data is the averaged value obtained from triplicate cultivations. The error bars are not included for clarity.