Figure 3.

Local treatment of the axonal microenvironment with inflammatory cytokine IL-6 enhances capsaicin-evoked responsiveness. (A) Representative soma images in short-term experiments of calcium responses to 100 nM capsaicin applied in the axonal chamber. (B) Quantification of the number of positive responders as a proportion of total neurons post-exposure to 100 nM capsaicin in vehicle and IL-6 treated groups, where * denotes test of proportions, p = 0.02 (C) Representative traces of baseline-normalized fluorescence intensity of calcium influx post-axonal stimulation as a function of time from defined regions of interest. (D) Magnitude of the calcium transient recorded post-exposure to 100 nM capsaicin in vehicle- and IL-6-treated groups, where *** denotes two sample unpaired t-test, p < 0.0001.