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. 2021 Nov 16;26(22):6897. doi: 10.3390/molecules26226897

Figure 3.

Figure 3

Alkaline phosphatase enzymatic activity, total protein amount and distribution of raft and non-raft markers in lipid raft and non-lipid raft fractions isolated from frontal cortical synaptosomes derived from wild-type mice. (A) Representative Western blots running in parallel same volume (20 µL/lane) of the collected 12 fractions and of the pellet (P). Immunoblot against Na+/K+-ATPase, Flotillin, Thymocyte (Thy-1), CB1 receptor, and Gαi/o subtypes. Protein migration was consistent with their expected molecular mass. For the CB1 receptor and the Gαi2 protein, an extra band migrating at ~35 kDa and ~36 kDa was detected, respectively. Na+/K+-ATPase, 112.3 kDa; Flotillin, 47.5 kDa; thymocyte 1 (Thy-1), 18.1 kDa; CB1 receptor, 52.8 kDa; Gαo 40.1 kDa; Gαi1, 40.5 kDa; Gαi2, 40.4 kDa; Gαi3, 40.5 kDa. The molecular weights depicted correspond to the signal of the standard markers. (B) Total protein content of the collected 12 fractions and of the pellet (P). (C) Alkaline phosphatase activity of the collected 12 fractions and of the pellet (P).