Table 1.
Concentration–response curves for agonist-stimulated specific [35S]GTPγS binding in frontal cortical and hippocampal synaptosomes derived from CB1-RS, Glu-CB1-RS, and GABA-CB1-RS mice. Values correspond to the means ± SEM of three independent experiments. Both in frontal cortex and hippocampus the experiments were carried out using two preparations enriched in synaptosomes, each of them obtained from pools of the frontal cortices and hippocampi of eight adult mice. Unpaired (%Emax) or paired (pEC50, Basal) one-way ANOVA followed by sidak test.
| CB1-RS | Glu-CB1-RS | GABA-CB1-RS | |
|---|---|---|---|
| Frontal Cortex | |||
| CP 55,940 | |||
| %Emax | 211.7 ± 2.37 | 157.11 ± 1.79 * | 159.00 ± 1.10 * |
| pEC50 | 6.85 ± 0.07 | 6.77 ± 0.12 | 6.72 ± 0.12 |
| WIN 55,221-2 | |||
| %Emax | 248.0 ± 18.74 | 189.55 ± 12.17 * | 184.8 ± 6.39 * |
| pEC50 | 6.75 ± 0.11 | 6.14 ± 0.12 | 6.13 ± 0.04 |
| Hippocampus | |||
| CP 55,940 | |||
| %Emax | 248.60 ± 16.36 | 183.43 ± 14.4 * | 240.60 ± 10.67 # |
| pEC50 | 6.61 ± 0.09 | 6.59 ± 0.16 | 6.61 ± 0.09 |
| WIN 55,221-2 | |||
| %Emax | 270.90 ± 9.68 | 195.85 ± 5.44 * | 258.1 ± 12.70 |
| pEC50 | 6.11 ± 0.12 | 5.93 ± 0.13 | 6.02 ± 0.12 |
| Basal (cpm) | 28,040 ± 2102 | 23,344 ± 1767 * | 23,559 ± 1725 * |
* = significantly different from CB1-RS, p < 0.05; # = significantly different from Glu-CB1-RS, p < 0.05.