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. 2021 Nov 19;10(11):3249. doi: 10.3390/cells10113249

Figure 4.

Figure 4

Inhibition of class I HDACs is involved in up-regulation of insulin gene expression. (A) Butyrate dose-dependently reduces in vitro HDAC enzyme activity in a HDAC fluorometric assay. (B) NPICCs were treated with 1000 µM butyrate and HDAC activity was measured by HDAC Activity Fluorometric Assay. Butyrate (1000 µM) and trichostatin A (TSA, 1 µM) significantly inhibited HDAC enzyme activity. (C) NPICCs were treated with different HDAC inhibitors for 6 days. TSA (1 µM) (class I and class II inhibitor), mocetinostat (1 µM) and MS275 (1 µM) (selective class I inhibitors) but not TMP269 (1 μM) or MC1568 (1 μM) (selective class II inhibitors) significantly increased insulin gene expression. NPICCs cultured in B-IC medium were used as controls. Data from three to five independent experiments are presented as mean ± SD. * p  <  0.05, ** p  <  0.01, *** p < 0.001, **** p < 0.0001 vs. control groups.