Table 1.
Primer sequences for quantitative real time-PCR.
| Gene | Primer Sequence 5′-3′ | Accession No. |
|---|---|---|
| INS | Forward: CAGGCCTTCGTGAACCAG | NM_001109772.1 |
| Reverse: CTTGGGCGTGTAGAAGAAGC | ||
| GCG | Forward: GAATTCATTGCTTGGCTGGT | NM_214324.1 |
| Reverse: CATCTGAGAAGGAGCCATCAG | ||
| PDX1 | Forward: GTGGAAAAAGGAGGAGGACA | NM_001141984.3 |
| Reverse: CAGCTCCTCTCCCGAGGT | ||
| NKX6.1 | Forward: GCCTACCCCGTTTCAGTAGC | XM_021101796.1 |
| Reverse: GGGTGGACTCTGCATCACTC | ||
| NGN3 | Forward: GCCTGCGTCTCAGCTGAACTT | XM_021072424.1 |
| Reverse: AGCCAGAGGCAGGAGGAACAA | ||
| ALDOB | Forward: ATTTGGAGGGCACTCTGTTG | XM_021066854.1 |
| Reverse: AGGTTGATAGCATTGAGGTTGAG | ||
| GPR43 | Forward: TCATGGGTTTCGGCTTCTACAG | EU122439.1 |
| Reverse: GTACTGAACGATGAACACGACG | ||
| GPR41 | Forward: ACTACTTCTCATCCTCGGGGTT | JX566879.1 |
| Reverse: CTCCACTTCGCTCTTCTTCAGT | ||
| GAPDH | Forward: GTCGGTTGTGGATCTGACCT | NM_001206359.1 |
| Reverse: GTCCTCAGTGTAGCCCAGGA |
INS: insulin; GCG: glucagon; PDX1: pancreatic and duodenal homeobox 1; NGN3: neurogenin 3; NKX6.1: NK6 Homeo-box 1; ALDOB: aldolase fructose-bisphosphate B; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; GPR43: G-Protein Coupled receptor 43; GPR41: G-Protein Coupled receptor 41.