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. 2021 Nov 4;57(11):1203. doi: 10.3390/medicina57111203

Figure 5.

Figure 5

The autophagic degradation of SIRT1 in OA chondrocytes. (A) Characteristic immunoblot showing SIRT1 levels under HCQ treatment in OA chondrocytes. Antibody against β-actin was used as loading control. (B) Graph demonstrating SIRT1 levels based on band density calculated using ImageJ. Values shown are the means ± S.E. ** p < 0.01 vs. the OA under normal conditions. (C) IP of extracts from normal and OA chondrocytes treated or not with HCQ. Excessive beads and antibodies were used in IP to capture nearly 100% of SIRT1 protein in the lysates. The Western blot from input is used as indicator of the existence of SIRT1 and LC3-I/II in extracts. (D) Graph demonstrating LC3-II IP levels normalized to the SIRT1 IP bands on normal chondrocytes treated or not with HCQ. Band density calculated using ImageJ. (E) Graph demonstrating LC3-II IP levels normalized to the SIRT1 IP bands on OA chondrocytes treated or not with HCQ. Band density calculated using ImageJ. Values shown are the means ± S.E. ** p < 0.01 vs. the LC3-II/SIRT1 in OA chondrocytes under normal conditions.