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. 2000 Feb;38(2):830–838. doi: 10.1128/jcm.38.2.830-838.2000

FIG. 1.

FIG. 1

FIG. 1

FIG. 1

Fluorescence microscopy of microorganisms after FISH with various oligonucleotide probes. (A) S. epidermidis (left) and S. aureus (right) were stained with DAPI (blue signal; staining of DNA) following FISH with probes Sta-185-FITC (green signal; specific for all staphylococci) and Sau-69-Cy3 (red signal; specific for S. aureus). (B) E. coli, K. pneumoniae, P. aeruginosa, and S. maltophilia (vertical columns) were stained with DAPI (blue signal; staining of DNA) following FISH with probes Eub-338-FITC (green signal; specific for all Eubacteria) and Ent-186 (specific for all Enterobacteriaceae), Kpn-1707 (specific for K. pneumoniae), Pae-1506 (specific for P. aeruginosa), and Sma-633 (specific for S. maltophilia), each Cy3-labelled (red signal). (C) C. albicans, C. glabrata, C. krusei, and C. parapsilosis (vertical columns) were stained with DAPI (blue signal; staining of DNA) following FISH with probes PF-2-FITC (green signal; specific for all yeasts) and Calb-1249 (specific for C. albicans), Cagl-651 (specific for C. glabrata), Ckru-1453 (specific for C. krusei), and Cpara-651 (specific for C. parapsilosis), each Cy3 labelled (red signal).