TABLE 3.

Results of examination of blood cultures with positive growth indices by Gram stain, FISH, and culture^a

Gram stain examination results		FISH results		Culture results
Morphology	n	Oligonucleotide	n	Genus or species	n
Gram-positive cocci (clusters)	62	Eubacteria	62	Eubacteria	62
		Controlb	0
		Staphylococcus spp.	62	Staphylococcus spp.	62
		Non-S. aureus	41	Coagulase-negative Staphylococcic	41
		S. aureus	21	S. aureus	21
Gram-positive cocci (chains and pairs)	20	Eubacteria	20	Eubacteria	20
		Control	0
		Streptococcus spp.	19	Streptococcus spp.	19
		S. pyogenes	0	S. pyogenes	0
		S. agalactiae	0	S. agalactiae	0
		Enterococcus spp.	8	Enterococcus spp.	8
		S. pneumoniae	7	S. pneumoniae	7
				Viridans group streptococcid	4
		Not identified	1	L. lactis	1
Gram-negative rods	30	Eubacteria	30	Eubacteria	30
		Control	0
		Enterobacteriacea	23	Enterobacteriaceae	23e
		K. pneumoniae	2	K. pneumoniae	2
		P. aeruginosa	3	P. aeruginosa	3
		S. maltophilia	0	S. maltophilia	0
		Not identified	2	M. osloensis	1
				B. fragilis	1
Yeasts	2	All yeasts	2	Yeasts	2
		Control	0
		C. albicans	2	C. albicans	2
		C. glabrata	0	C. glabrata	0
		C. parapsilosis	0	C. parapsilosis	0
		C. krusei	0	C. krusei	0
Others	1	Eubacteria	1	Propionibacterium spp.	1
		Control	0

^aA total of 115 blood culture specimens were analyzed by FISH. Culture was performed on agar plates, and microorganisms were identified by standard laboratory methods (see Materials and Methods). 

^bA nonsense control oligonucleotide was used (see Materials and Methods). 

^cCoagulase-negative staphylocci included S. epidermidis, S. sciuri, S. haemolyticus, S. schleiferi, and S. cohnii. 

^dViridans group streptococci were identified by binding of the Streptococcus genus probe detecting all streptococci and simultaneous exclusion of S. pyogenes, S. agalactiae, Enterococcus spp., and S. pneumoniae. 

^eIncluding E. coli, K. pneumoniae, K. oxytoca, C. freundii, E. aerogenes, and E. cloacae.