Table 1.

Effect of dark incubation periods, Parafilm wrapping, and the interaction on shoot regeneration. Decapitated seedlings wrapped with or without Parafilm were kept in the dark for 0, 5, 10, 15, and 20 days prior to culture under natural light conditions for another 2 weeks.

Treatments		With Parafilm	Without Parafilm	Mean Factor B (Dark Incubation)
Dark incubation periods	0 day	37.33 ± 3.89 d	9.33 ± 4.42 e	23.33 ± 15.39 d
	5 days	47.33 ± 9.04 c	17.33 ± 4.90 e	32.33 ± 17.57 c
	10 days	70.67 ± 6.46 b	36.00 ± 4.42 d	53.33 ± 19.18 b
	15 days	90.00 ± 5.96 a	41.33 ± 5.42 c d	65.67 ± 26.34 a
	20 days	87.33 ± 6.46 a	48.67 ± 6.86 c	68.00 ± 21.55 a
	Mean Factor A (Parafilm)	66.53 ± 22.53 a	30.53 ± 16.06 b
LSD Factor A (p ≤ 0.05)		7.025
LSD Factor B (p ≤ 0.05)		5.832
LSD Interaction A × B (p ≤ 0.05)		8.248

Note: data are represented as mean ± SD (standard deviation) of regenerated/total seedling. The different letters in this table mean significant at p ≤ 0.05 level using LSD Test.