Table 2.

In vitro inhibitory effects of final compounds.

Compounds	IC50 a	PfCK Fractional Activity at 25 µM	IC50 PfCK b
Fa-M2	0.0489	100.75 $\pm 13.73$
Fa-M1	0.0410	54.65 $\pm 11.69$
Fa-M3	0.0351	96.91 $\pm 18.68$
Fg-9	0.1659	nd
Fa-21	0.0137	nd
Fg-14	0.0206	nd
Fa-24	0.0578	57.08 ± 5.15
Fg-30	0.0279	54.77 ± 7.20
Fg-10	0.0530	44.66 $\pm 8.55$
Fa-22	0.0514	57.03 $\pm 5.37$	11.70 ± 0.49
Fg-18	0.0604	49.53 $\pm 8.06$	11.09 ± 1.00
Fp-1	0.1422	12.88 $\pm 3.32$	7.27 ± 0.23
Fp-8	0.2816	8.10 $\pm 5.80$	4.17 ± 0.48
Fg-12	0.3049	nd
Fg-17	0.2273	50.28 $\pm 7.88$
Fg-13	0.1429	99.13 $\pm 12.01$
Fa-27	0.1205	82.64 $\pm 4.90$
Fg-32	>0.25	83.85 $\pm 9.53$
Fa-26	0.2093	78.02 $\pm 8.13$
Fg-20	0.0976	96.62 $\pm 13.12$
Fg-11	0.2321	32.96 $\pm 1.48$	16.46 ± 1.43
Fg-16	0.0206	79.74 $\pm 10.35$	26.53 ± 2.44
Fg-15	0.0127	97.48 $\pm 9.22$
Fa-25	0.0347	nd
Fg-31	0.0347	87.41 $\pm 2.20$
Fa-23	0.0678	nd
Fg-19	0.0715	nd
Ff-1	0.0901	47.03 $\pm 3.53$	10.90 ± 1.33
Ff-7	0.0219	55.02 $\pm 4.07$	13.32 ± 3.82
Ff-3	0.0388	nd
Fa-33	0.0439	68.76 $\pm 6.03$
Ff-6	nd	nd
Fa-29	0.0556	46.72 $\pm 9.49$	4.64 ± 0.20
Ff-35	0.0484	57.62 $\pm 12.29$
Ff-2	0.0740	81.77$\pm 3.93$
Ff-8	0.0352	63.06 $\pm 6.74$
Ff-4	0.0115	90.72 $\pm$ 4.57	37.36 ± 3.24
Fa-28	0.0471	84.39 $\pm 7.53$
Ff-5	0.0224	91.06 $\pm 4.27$
Ff-34	0.0879	52.17 $\pm 12.43$
Ff-36	0.1861	46.39 $\pm 12.44$
Reference Compounds c
HC-3	>50		250
MN58b	0.0035		106
RSM-932A	0.0265		1.75
BR-23	0.003 ± 0.0002		276
BR-25	0.002 ± 0.0003		103
BR-31	0.007 ± 0.0006
BR-33	0.045 ± 0.003
10a	0.016 ± 0.001		>≥100
10k	0.035 ± 0.004		10.949 ± 0.124
Chloroquine	0.015 ± 0.2709

^a In vitro IC50 values against P. falciparum (μM). ^b IC50 = Compound concentration required to inhibit PfCK enzyme by 50% (μM). nd: not determined. ^c Data from Refs. [7,8,10].