Figure 3.

PCR confirmation of the recombinant LSDV-BEFV candidate vaccines. DNA was extracted from infected MDBK cells and subjected to PCR using forward (for) and reverse (rev) primers as indicated in Figure 2. Fragments were separated by electrophoresis on 0.8% agarose gels. M—GeneRuler 1 kb DNA Ladder (Thermo Fisher Scientific), lanes 1 = vaccine of interest, as labelled above or below the images; lanes 2 = parent LSDV (nLSDV∆SOD-UCT or nLSDVSODis-UCT) and lanes 3 = uninfected cells.