Table 1.
Summary of components used in the construction of six different LSDV-BEFV dual vaccines.
| Vaccine | LSDV Parent Virus | Genes Inserted between LSDV ORFS 49 and 50 * | Marker * |
|---|---|---|---|
| nLSDV∆SOD-UCT | Neethling LSDV vaccine with SOD homolog gene (ORF 131) deleted [29] | parent virus with no gene insertions | None |
| nLSDVSODis-UCT | Neethling LSDV vaccine with full length modified SOD homolog gene (ORF 131) [29] | parent virus with no gene insertions | None |
| LSDV(∆SOD)BEFV-Ga | nLSDV∆SOD-UCT | BEFV glycoprotein gene of Australian origin (Ga) [14] | mCherry |
| LSDV(SODis)BEFV-Ga | nLSDVSODis-UCT | ||
| LSDV(∆SOD)BEFV-Gb | nLSDV∆SOD-UCT | BEFV glycoprotein gene based on consensus sequence of South African G protein genes (Gb) [18] | eGFP |
| LSDV(SODis)BEFV-Gb | nLSDVSODis-UCT | ||
| LSDV(∆SOD)BEFV-Gb-M | nLSDV∆SOD-UCT | BEFV glycoprotein gene based on consensus sequence of South African G protein genes (Gb) [18] and matrix gene (M) [19] | eGFP |
| LSDV(SODis)BEFV-Gb-M | nLSDVSODis-UCT |
* Both BEFV glycoprotein genes (Ga [14] and Gb [18]) are under the control of the vaccinia virus mH5 promoter [33], the BEFV matrix [19] and mCherry genes are controlled by the modified fowlpoxvirus (mFPV) promoter [34], and the eGFP gene is controlled by the synthetic vaccinia virus promoter (pS) [35,36].