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. 2021 Oct 30;12(11):1747. doi: 10.3390/genes12111747

Figure 5.

Figure 5

Effect of overexpression of WTAP on cell proliferation. (A) Western blot analysis of WTAP expressed in WTAP-OE and CON-OE cells. (B) RT-qPCR analysis of WTAP transcripts from WTAP-OE and CON-OE cells. (C) CCK-8 analysis was made to examine cell proliferation. (D) Colony formation analysis of WTAP-OE cells and CON-OE cells treated with different concentrations of RAPA. (E) Statistical analysis of cell survival of WTAP-OE cells and CON-OE treated with RAPA. (F) Statistical analysis of cell viability of WTAP-OE cells and CON-OE treated with RAPA. Error bars indicate the mean ± SD from three independent experiments (n = 3). ** indicate a statistically significant difference (p < 0.01), and *** indicates a statistically significant difference (p < 0.001).