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Ferric reducing antioxidant power (FRAP) assay of OESA. Various concentrations of OESA extracts (0 to 1 mg/mL) and acid ascorbic were mixed with FRAP reagents. The reduction of ferric ion (Fe3+) to ferrous form (Fe2+) by OESA produces an intense blue light revealed as a change in absorption at 700 nm. Results were expressed as mean inhibition percentage (%) ± standard deviations (n = 3). Ascorbic acid at various concentrations (0 to 1 mg/mL) was used as standard.
