Table 5.

Environmental factors-induced DED model and experiment schedule.

Animal	Humidity	Airflow	Temperature	Time of Induction	Starting of Treatment	End of Experiment	References
C57BL/6 mice	13.1% ± 3.5%	2.2 ± 0.2 m/s	22 ± 2 °C	21 days	Beginning of housing in the ICES or on day 22 after DED confirmation. 10 µL topically, four times daily until 35 days.	35 days	Chen et al. [115] 2013
BALB/c mice	13.1% ± 3.5%	2.2 ± 0.2 m/s	22 ± 2 °C	21 days	After 21 days housed in the ICES, the mice were administered 10 μ of eye drops, four times daily (every 6 h) for 14 days during which the mice remained housed in the ICES.	35 days	Chen et al. [116] 2009
C57BL/6 mice	13.1% ± 3.5%	2.2 ± 0.2 m/s	22 ± 2 °C	21 days	10 μL/eye bilaterally four times a day for 3 weeks.	After 3 weeks	Li et al. [117] 2012
C57BL/6 mice	15.3% ± 3.0%	2.1 ± 0.2 m/s	21 °C ± 2 °C	14 days	Beginning of housing in the ICES or on day 14 after DED confirmation. 10 µL topically, 4 times daily until 28 days	28 days	She et al. [109] 2015
BALB/c mice	15.3% ± 3%	2.1 ± 0.2 m/s	21 ± 3 °C	42 days	–	42 days	Chen et al. [110] 2008
C57BL/6 mice	13.1 ± 3.5%, airflow and temperature	2.2 ± 0.2 m/s	22 ± 2 °C	An alternating 12 h light–dark cycle (8:00 a.m. to 8:00 p.m.) was employed for 1, 2, 4, and 6 weeks		1, 2, 4, and 6 weeks	Xiao et al. [93] 2015
Mice	Topical application of:00 PM2.5. 5.0 mg/mL, 4 times daily					4, 7, 10, and 14 days	Tan et al. [113] 2018
Mice	Topical application of PM10 5.0 mg/mL to right eyes, 4 times daily.					At 14 days	Li et al. [114] 2017
Rats	Rats were exposed to approximately 500 μg/m3 UPM in the exposure chamber for 5 h per day over 5 days.					5 days after exposure of UPM	Song et al. [112] 2020