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. 2021 Nov 20;13(11):1967. doi: 10.3390/pharmaceutics13111967

Figure 2.

Figure 2

Figure 2

Construction of recombinant X-33-L1P18 yeast strain. (A) Cloning of L1P18 DNA coding sequence into yeast plasmid DNA pGAPZB. (B) PCR analysis of genomic DNA isolated from recombinant P. pastoris clones. Amplification of a 1563-bp DNA fragment containing L1P18 reveals positive clones. Lane 1, molecular weight markers; lane 2, Recombinant P. pastoris X-33 harboring the pGAPZB without heterologous DNA insert (negative control); lane 3, P. pastoris X-33 harboring the pGAPZB-L1P18 expressing the gene encoding the L1P18 protein; lane 4, pGAPZB-L1P18 plasmid (positive control) (C) Western blot analysis of recombinant P. pastoris cell lysate. Lane 1, molecular weight markers (50 kDa and 60 kDa); lane 2, Recombinant P. pastoris X-33 harboring the pGAPZB without heterologous DNA insert (negative control); lane 3, P. pastoris X-33 harboring the pGAPZB-L1P18 expressing the gene encoding the L1P18 protein; lane 4, abcam HPV16 L1 protein 100 ng (positive control, 56 kDa).