Figure 6.

Vaccinated mice challenged with CVB4 did not reveal detection of viral nucleic acids. Groups of mice were given saline or were vaccinated with Mt10 virus, and 14 days later, challenged with or without CVB4. Hearts and pancreata were collected three weeks post-challenge, total RNA was extracted, and viral RNA was estimated by qPCR targeting VP1 sequences. After normalizing the expression levels of viral RNA relative to GAPDH, 2^−(ΔΔCt) values were calculated. Mean ± SEM values representing four samples per group, each containing n = 3–5 mice, are shown. Mann–Whitney test was used to determine significance between groups. * p ≤ 0.05; ns—not significant.