Figure 2.

Effect of Cr and NAM pretreatment on oxidant-induced β-gal activity. DHF were exposed to various concentrations of Cr or NAM alone or combined (5 mM each), media alone (SHAM), or water vehicle (1%). Twenty-four hours later, the cells were exposed to 600 µM H₂O₂ for 2 h, followed by replacement with treatment and harvesting 72 h later for β-gal staining. (A) representative pictures of β-gal staining of vehicle versus H₂O₂ treatment, indicating increased staining (senescence) due to oxidant exposure; (B,C) % β-gal positive cells in response to various treatments. The data are mean ± SE from 3–4 separate experiments using duplicate samples. * Denotes statistically significant changes in the Cr/NAM treated levels in comparison to H₂O₂ alone. NS, the treatments compared to one another are not statistically significantly different.