Figure 3.

Effect of Cr and NAM on oxidant induced p21 levels. DHF were exposed to various concentrations of 10 mM Cr or 2.5 or 5 mM NAM alone or combined (5 mM each), media alone (SHAM), or water vehicle (1%). Twety-four hours later, the cells were exposed to 600 µM H₂O₂ for 2 h, followed by harvesting 72 h later for p21 protein analysis. (A,B) representative Western blotting indicating that H₂O₂ treatment increased p21 protein expression, whereas pretreatment with (A) Cr and (B) NAM did not result in increased p21 expression levels; (C) graphical summary of Western blot images normalized to actin. The data are mean ± SE from 3–4 separate experiments using duplicate samples. Data sets of p21 levels were quantified relative to actin and normalized to an H₂O₂ group. * Denotes statistically significant changes in the Cr/NAM treated levels in comparison to H₂O₂ alone. NS, the treatments compared to one another are not statistically significantly different.