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The effect of pH and temperature on the recovery of PEF-injured S. cerevisiae. Survival fraction of S. cerevisiae after the PEF treatment (20 kV/cm for 200 μs) in citrate-phosphate buffer at pH 4.0 (A,C) and pH 7.0 (B,D), and in the subsequent recovery process incubated at 30 °C (A,B) or 4 °C (C,D) for 4 h. YPD, non-selective medium; YPD-SC, selective medium. Data are expressed as mean ± standard deviation. Significant differences are indicated by different letters (p < 0.05).
