Table 1.
Primers used for RT-qPCR.
| Gene Name | Gene Bank Accession Number | Primer Sequence (5′–3′) |
Annealing Temperature [°C] | Amplicon Length [bp] |
|---|---|---|---|---|
| HPRT1 | NM_000194.3 | F: ATGGACAGGACTGAACGTCTT R: TGTAATCCAGCAGGTCAGCA |
57 | 118 |
| PPIA | NM_021130.5 | F: GCCGAGGAAAACCGTGTACT R: CTGCAAACAGCTCAAAGGAGAC |
59 | 106 |
| SGLT1 | NM_000343.4 | F: AAGACCACCGCGGTCAC R: AAACATAGCCCACAGTCCGA |
57 | 119 |
| GLUT1 | NM_006516.4 | F: GAACTCTTCAGCCAGGGTCC | 60 | 113 |
| R: ACCACACAGTTGCTCCACAT | ||||
| GLUT2 | NM_000340.2 | F: CAATGCACCTCAACAGGTAATAA R: AGATTGTGGGCAGTTCATCTGT |
57 | 119 |
| PEPT1 | NG_017032.1 | F: GGTCCCACTGCTGCTTTTTG | 60 | 117 |
| R: GCAGAGCAAGGGGTTGTACT | ||||
| EAAT1 | NM_001166696.3 | F: CCCCTTACAAAATCAGAAAAGTTGT | 60 | 101 |
| R: GGAGGGTCTCTTCTTTGCACT | ||||
| EAAT3 | NM_004170.6 | F: GGTGGTGCTAGGCATTACCA | 60 | 118 |
| R: TGATGAGTTTCAGCATCCGCA | ||||
| ATB0 | NM_005628.3 | F: GTGTCCTCACTCTGGCCATC | 60 | 98 |
| R: TACAGGACCGGTCGACTAGC | ||||
| VILLIN | NM_007127.3 | F: ACCGAGGGGATGTTTTCCTC R: GTCATGCCCCTGAGTCTCTC |
60 | 93 |
| CLD 1 | NM_021101.5 | F: CCAGTCAATGCCAGGTACGA | 60 | 93 |
| R: ACAGCAAAGTAGGGCACCTC | ||||
| CLD 4 | NM_001305.5 | F: TCTCCTCTGTTCCGGGTAGG | 60 | 90 |
| R: CAAGGCCTCAGCCATACTCC | ||||
| OCLN | NM_002538.4 | F: GGTCTAGGACGCAGCAGATTG | 60 | 112 |
| R: TGGACTTTCAAGAGGCCTGG |
F: forward primer; R: reverse primer; bp: base pairs; HPRT1: hypoxanthine guanine phosphoribosyltransferase 1, PPIA: peptidylprol-yl-isomerase A, SGLT1: Na+-coupled glucose transporter 1, GLUT: glucose transporter, PEPT1: peptide transporter 1, EAAT: excitatory amino acid transporter, ATB0: amino acid transporter B0, CLD: claudin, OCLN: occludin.