Table 2.
Antibodies used for Western Blot.
| Target | Primary Antibody | Manufacturer/Catalog Number | Dilution | Secondary Antibody | Manufacturer/ Catalog Number |
Dilution |
|---|---|---|---|---|---|---|
| β-ACTIN | Mouse-anti-β-Actin | Santa Cruz Biotechnology/sc-47778 | 1:1000 | Goat-anti-mouse HRP | Invitrogen/A16072 | 1:5000 |
| DPPIV | Mouse-anti-Dipeptidyl peptidase (HBB 3/775/42) | Provided by Dres. E.E. Sterchi and H.-P. Hauri. University of Bern and University of Basel, Switzerland [23,24] | 1:5000 | Goat-anti-mouse HRP | Invitrogen/A16072 | 1:5000 |
| GLUT2 | Rabbit-anti-GLLUT2 | Thermo Fisher Scientific/PA5-77459 | 1:200 | Donkey-anti-rabbit HRP | Santa Cruz Biotechnology/sc-2077 | 1:10,000 |
| Na+/K+-ATPase | Mouse-anti-Na+/K+-ATPase | Enzo Life Sciences AG 804-082 | 1:100 | Goat-anti-mouse HRP | Invitrogen/A16072 | 1:5000 |
| SGLT1 | Rabbit-anti-SGLT1 | Antibodies online/ABIN364451 | 1:1000 | Donkey-anti-rabbit HRP | Santa Cruz Biotechnology/sc-2077 | 1:10,000 |
| SI | Mouse-anti-sucrase–isomaltase (HBB3/705/60) | Provided by Drs. E.E. Sterchi and H.-P. Hauri. University of Bern and University of Basel, Switzerland [23,24] | 1:5000 | Goat-anti-mouse HRP | Invitrogen/A16072 | 1:5000 |
| VILLIN | Rabbit-anti-Villin | Thermo Fisher Scientific/PA5-78222 | 1:500 | Donkey-anti-rabbit HRP | Santa Cruz Biotechnology/sc-2077 | 1:10,000 |
| FLOTILLIN-2 | Mouse-anti-flotillin-2 B-6 | Santa Cruz Biotechnology/sc-28320 | 1:5000 | Goat-anti-mouse HRP | Santa Cruz Biotechnology, Inc., Texas, US | 1:5000 |
DPPIV: dipeptidyl-peptidase IV, GLUT2: glucose transporter 2, SGLT1: Na+-coupled glucose transporter 1, SI: sucrase-isomaltase.