Table 3.
Determination of transgene copy numbers of T0 and T4 leaf tissues using droplet digital PCR.
| Event |
Alk Copy/µL |
Adh1 Copy/µL |
Alk/Adh1 | Copy Number * |
|---|---|---|---|---|
| Alk-3 (T0) | 330 | 302 | 1.09 | 1 (hemi) |
| Alk-4 (T0) | 563 | 603 | 0.93 | 1(hemi) |
| Alk-6 (T0) | 238 | 245 | 0.97 | 1 (hemi) |
| Alk-7 (T0) | 85.9 | 48.6 | 1.76 | 2 (hemi) |
| Alk-9 (T0) | 540 | 249 | 2.17 | 2 (hemi) |
| Alk-10 (T0) | 593 | 274 | 2.16 | 2 (hemi) |
| Alk-11 (T0) | 187 | 181 | 1.03 | 1 (hemi) |
| Alk-3 (T4) | 342 | 153.7 | 2.23 | 1 (homo) |
| Alk-4 (T4) | 529 | 290 | 1.82 | 1 (homo) |
| Alk-7 (T4) | 144.4 | 36.7 | 4.20 | 2 (homo) |
| Alk-9 (T4) | 256.8 | 54.9 | 4.68 | 2 (homo) |
* The target gene copy number was calculated based on the ratio between number of molecules of target alk gene and maize single copy reference alcohol dehydrogenase gene (adh1) in the genomic DNA samples. The primers for alk gene used in the ddPCR has one target in each of two alk arms in the construct.