Figure 6.

FAD2 and FATB candidate genes in soybean and its sgRNA sequence and target location. (A) sgRNAs and their sequence. (B) Based on transcript form, left white box for 5′ end UTR, right white box for 3′ end UTR, red box for exon, angled line for intron splicing, and colored triangle for sgRNA target sites. Black asterisk in front of gene accession number indicates major expressing genes. Red asterisk on colored triangle indicates one base pair mismatch between gDNA sequence and sgRNA sequence. This mismatch is also indicated in red letters in the sgRNA4, sgRNA5, and sgRNA6 sequences in Figure 7A.