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Gel picture of PCR or ligation products. (A) PCR product of insert fragment. Each fragment numbers indicate sequential order. 1: DT1 and DT2 primer set used fragment, 2: DT3 primer set used fragment, 3: DT4 primer set used fragment. (B) Product of Golden Gate reaction. +indicate ligation of insert fragment. (C) Product of pHEE401E vector colony PCR. Yellow asterisk indicated by the expected band is the expected result.
