Table 2.

List of primers and sequences for the pBAtC_4sg insert.

Insert Name	Sequence
Bb_g1g2_F1	(--sg1--)GTTTTAGAGCTAGAA
Bb_g1g2_F2	ATGAAGACNNTGCA(--sg1--)
Aa_g1g2_R	ATCACCTGCNNNN(--half of sg2, 5′ moiety--)TGCACCAGCCGGGAA
Aa_g2g3_F	ATCACCTGCNNNN(--half of sg2, 3′ moiety--)GTTTTAGAGCTAGAAATAGC
Aa_g2g3_R	ATCACCTGCNNNN(--half of sg3, 5′ moiety--)TGCACCAGCCGGGAATCGAA
Aa_g3g4_F	ATCACCTGCNNNN(--half of sg3, 3′ moiety--)GTTTTAGAGCTAGAA
Bb_g3g4_R1	(--sg4--)TGCACCAGCCGGGAATCGAA
Bb_g3g4_R2	ATGAAGACNNAAAC(--sg4--)

Underlined sequences are restriction-enzyme-recognition sites.