Table 5.

Golden Gate reaction components and thermal conditions.

Item	Concentration	Amount	Temperature	Time
Insert 1		(300 ng)	37 °C	5 h
Insert 2		(300 ng)	50 °C	5 min
10X T4 ligase Buffer		1.5 μL	80 °C	10 min
BbsI	10 U/μL	1 μL	8 °C	Hold
T4 Ligase	350 U/μL	1 μL
Distilled Water		to 15 μL