Table 16.

List of primers for pBAtC_4sg insert fragments.

Insert Name	Sequence
Bb_g1g2_F1	GTGTTTGGAACCCTTGAGAGGTTTTAGAGCTAGAA
Bb_g1g2_F2	ATGAAGACATTGCAGTGTTTGGAACCCTTGAGAG
Aa_g1g2_R	ATCACCTGCATATTGTTCCTTCATCTGCACCAGCCGGGAA
Aa_g2g3_F	ATCACCTGCATATAACATCCGAGAAGTTTTAGAGCTAGAAATAGC
Aa_g2g3_R	ATCACCTGCATATTGGAGCAGGCAGTGCACCAGCCGGGAATCGAA
Aa_g3g4_F	ATCACCTGCATATTCCACCAGAGTCGTTTTAGAGCTAGAA
Bb_g3g4_R1	TTAATTTTCAAAGGGGCTTGTGCACCAGCCGGGAATCGAA
Bb_g3g4_R2	ATGAAGACATAAACTTAATTTTCAAAGGGGCTTG

Sticky ends that were generated by type IIS restriction enzyme are indicated with red text. Underlined sequences are restriction-enzyme-recognition sites.