Antibodies |
|
|
CD3-biotin |
BD Biosciences |
Cat# 553060, RRID:AB_394593 |
TCR-biotin |
BD Biosciences |
Cat# 553168, RRID:AB_394680 |
B220-biotin |
BD Biosciences |
Cat# 553085, RRID:AB_394615 |
B220-BV785 |
BioLegend |
Cat# 103245, RRID:AB_11218795 |
B220-BV421 |
BioLegend |
Cat# 103239, RRID:AB_10933424 |
CD21-PerCP-CY5.5 |
BioLegend |
Cat# 123415, RRID:AB_1595595 |
CD23-FITC |
BD Biosciences |
Cat# 561146, RRID:AB_10611730 |
CD23-BUV737 |
BD Biosciences |
Cat# 564436, RRID:AB_2738806 |
AA4.1-BV650 |
BD Biosciences |
Cat# 740548, RRID:AB_2740250 |
CD45.1-BV650 |
BioLegend |
Cat# 110735, RRID:AB_11124743 |
CD45.2-FITC |
BD Biosciences |
Cat# 561874, RRID:AB_10894189 |
CD45.2-PE-TR |
BioLegend |
Cat# 405725, RRID:AB_2562743 |
CD95-biotin |
BD Biosciences |
Cat# 554256, RRID:AB_395328 |
GL7-PerCP-Cy5.5 |
BioLegend |
Cat# 144609, RRID:AB_2562978 |
IgD-BV421 |
BioLegend |
N/A |
IgM-PE-Cy7 |
BD Biosciences |
Cat# 552867, RRID:AB_394500 |
Ki67-FITC |
BD Biosciences |
Cat# 612472, RRID:AB_399649 |
Ki67-PE |
BD Biosciences |
Cat# 561283, RRID:AB_10716060 |
Chemicals, Peptides, and Recombinant Proteins |
|
|
Tamoxifen |
Sigma-Aldrich |
Cat# T5648 |
Busilvex® (Busulfan) |
Pierre Fabre |
N/A |
Critical Commercial Assays |
|
|
Streptavidin Dynabeads |
Life Technologies |
Cat# 11047. |
Live/dead Near-IR |
Life Technologies |
Cat# L34976. |
Streptavidin-BUV395 |
BD Biosciences |
Cat# 564176 |
Streptavidin-PerCP-Cy5.5 |
BioLegend |
Cat# 405214. |
Transcription factor staining buffer set |
BD Biosciences |
Cat# 562574 |
Experimental Models: Organisms/Strains |
|
|
C57Bl6/J |
UCL Comparative Biology Unit |
Strain 0159 |
SJL.C57Bl6/J |
UCL Comparative Biology Unit |
Strain A423 |
Mki67mCherry-CreERT2
Rosa26REYFP
|
UCL Comparative Biology Unit |
This study |
Rag2
GFP
|
The Jackson Laboratory |
Stock No. 005688. |
Software and Algorithms |
|
|
FlowJo Software |
FlowJo LLC |
N/A |
GraphPad Prism |
GraphPad Software, Inc |
N/A |
Custom R code used for modeling and data analysis |
https://github.com/sanketrane/B_cells_FM_GC
|
N/A |