Table 4.
Liver oxidative stress and antioxidants markers.
| Parameters | N | HFD | HFD + O | OB + O |
|---|---|---|---|---|
| TBARS (nmol/mg protein) | 2.47 ± 0.07 | 6.25 ± 0.61 a | 2.07 ± 0.27 b | 1.82 ± 0.11 b |
| NO (µmol/g protein) | 0.84 ± 0.04 | 1.20 ± 0.07 a | 0.92 ± 0.03 b | 0.91 ± 0.06 b |
| PCO (mmol/mg protein) | 0.80 ± 0.05 | 1.80 ± 0.07 a | 0.86 ±0.06 b | 0.95 ± 0.11 b |
| SOD (unit/mg protein) | 5.65 ± 0.27 | 2.12 ± 0.51 a | 5.08 ± 0.37 b | 4.09 ± 0.50 b |
| CAT (unit/mg protein) | 28.28 ± 3.56 | 6.70 ± 1.14 a | 16.45 ± 0.80 b | 21.77 ± 3.03 b |
| GPx (unit/mg protein) | 2.45 ± 0.20 | 0.79 ± 0.16 a | 2.416 ± 0.18 b | 1.79 ± 0.11 a,b |
| GST (unit/mg protein) | 5.49 ± 0.64 | 1.47 ± 0.09 a | 4.26 ± 0.34 b | 4.62 ± 0.62 b |
| GSH (nmol/mg protein) | 3.29 ± 0.08 | 2.37 ± 0.10 a | 3.06 ± 0.06 b | 2.91 ± 0.19 b |
| GR (unit/mg protein) | 15.36 ± 0.46 | 11.40 ± 0.36 a | 15.55 ± 0.60 b | 14.61 ± 0.87 b |
| TAC (nmol/mg protein) | 83.11 ± 2.59 | 65.92 ± 0.56 a | 84.11 ± 3.04 b | 83.70 ± 6.51 b |
Data are expressed as mean ± SEM, n = 6/group. N, normal control; HFD, high-fat diet; HFD + O, high-fat diet + orlistat 10 mg/kg/day (protective model); OB + O, obese + orlistat 10 mg/kg/day (therapeutic model); TBARS, thiobarbituric acid reactive substances; NO, nitric oxide; PCO, protein carbonyl; SOD, superoxide dismutase; CAT, catalase; GPx, glutathione peroxidase; GST, glutathione S-transferase; GSH, glutathione; GR, glutathione reductase; TAC, total antioxidant capacity. One-way ANOVA, followed by Tukey post-hoc test. a p < 0.05 vs. N group, b p < 0.05 vs. HFD group.