Figure 7.

Suppression effect of PSO (A) and RA-RF (B) on JNK phosphorylation. A549 cells were pretreated with PSO and RA-RF for 2 h and further incubated with tumor necrosis factor-α (TNF-α) for 30 min. Whole-cell lysates were analyzed by Western blotting with anti-p-JNK and anti-JNK antibodies. Actin was used as an internal control. All assays were performed in triplicate; data are presented as mean ± standard deviation (^## p < 0.01, ^### p <0.001 vs. the control group; * p < 0.05 *** p < 0.001 vs. (+) TNF-α induced group).