Figure 7.

Monitoring of [Ca²⁺]_cyt in response to hydrogen peroxide, nitrite, and nitrate at the same doses as those measured in the PAW at 1:2 dilution. Ca²⁺ measurement assays were conducted in aequorin-expressing Arabidopsis seedlings. At 100 s (arrowheads) seedlings were challenged separately with: (a) 8.7 µM H₂O₂ (green trace); (b) 520.1 µM NO₂⁻ (provided as K⁺ salt, yellow trace; provided as Na⁺ salt, orange trace); (c) 264.2 µM NO₃⁻ (provided as K⁺ salt, red trace; provided as Na⁺ salt, brown trace); and (d) a solution (mix) containing all the above chemicals (with NO₂⁻ and NO₃⁻ provided as K⁺ salt, grey trace; with NO₂⁻_and NO₃⁻ provided as Na⁺ salt, black trace). Data are the means (solid lines) ± SE (shading) of six different seedlings derived from three independent growth replicates.