Skip to main content
. 2021 Oct 20;13(11):2113. doi: 10.3390/v13112113

Figure 6.

Figure 6

Silencing of RhoB and Pak1 negatively impacts ZIKV infection. SNB-19 cells were treated with NT, and Rho GTPase- and Pak1-targeting siRNAs for 48 h and then infected with ZIKV (MOI = 0.1 PFU/cell) for 24 h. (A) mRNA levels of Rho GTPases and Pak1 were measured by RT-qPCR and normalized to that of NT siRNA-treated and ZIKV-infected cells. Significant knockdown was arbitrarily set at 75% (dotted line) to uncover potential effects conferred by Rho GTPases and effector proteins. (B) ZIKV RNA of cells treated with Rho GTPase-targeting siRNAs in (A) were quantified by RT-qPCR and normalized to that of NT siRNA-treated and ZIKV-infected cells (NT ZIKV). The data are from one experiment performed in triplicate. Asterisks indicate statistically significant differences (one-way ANOVA and Dunnett’s multiple comparisons test: *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001).