Figure 3.

Automated immunomagnetic enrichment procedure exhibits a significantly lower amount of leukocyte contamination compared to manual cell isolation. (A) Summary of enrichment processes and quantification of leukocyte contamination. (B–E) After the automated enrichment process, cells were stained with CD45-PE antibody and nucleic staining reagent RedDot^TM. Blood dilution (1:1000) shows a dual-positive cell population (B,C). After the enrichment process, this gating was used to quantify the leukocyte contamination (E) while showing no staining of the free beads themselves (D).