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. 2021 Oct 20;9(11):1213. doi: 10.3390/vaccines9111213

Figure 1.

Figure 1

Recombinant expression and purification of rLTB-FhTSP2, rFhTSP2, and rLTB. Yeast culture supernatant for (a) rLTB-FhTSP2, (b) rFhTSP2, and (c) rLTB were each applied to a Ni-NTA column and washed before elution with starter buffer containing 100–400 mM imidazole. A total of 15 µL of each pooled and concentrated nickel elutions (lane 1) and deglycosylated rLTB-FhTSP2 and rFhTSP2 after incubation with Endoglycosidase H (lane 2) was resolved by SDS-PAGE and stained with Coomassie blue. M, molecular weight markers.