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Characterisation of LTB oligomerisation. Both proteins rLTB-FhTSP2 (a,c) and rLTB (b,d) were loaded at 6 µg and subject to boiling at 95 °C for 10 min. Boiled (lane 1) and non-boiled (lane 2) proteins were analysied by SDS-PAGE and visualized by Coomassie staining (a,b) and Western blotting (c,d) using anti-His tag antibodies.
