Figure 3.

Characterisation of pentamer formation of the rLTB-FhTSP2 fusion complex via glycan receptor binding. Plates were coated with 3 μg/mL of GM1 ganglioside and blocked with 3% bovine serum albumin. Potential binding proteins (rEcxB and rLTB-FhTSP2) and negative control (rFhTSP2) were incubated in triplicate at three dilution factors (3, 0.3, and 0.03 μg/mL). Binding was detected by mouse anti-His tag HRP conjugated antibodies. All results are displayed as the mean of the adjusted optical density after subtraction of non-GM1 coated wells.