Skip to main content
. 2021 Nov 16;11(11):1237. doi: 10.3390/life11111237

Figure 3.

Figure 3

TSLP transcriptional regulator NFAT is not up-regulated in EGFR∆ep mice, and a NFAT pathway inhibitor does not block TSLP expression ex vivo. (A) NFAT expression was detected by immunofluorescence of 5-µm skin cryosections of WT and EGFR∆ep mice. Colours are as indicated in the pictures. (B) Epidermal LC network of epidermal sheets from skin explants treated with vehicle (EtOH) or tacrolimus (TAC). (C) Culture supernatant from WT skin explant cultures with EtOH (vehicle control) or tacrolimus (Tac) was collected after 24 h at indicated temperatures, and TSLP expression was detected by ELISA. Data represent means ± SEM. * p < 0.05. Data are from at least three independent experiments. Scale bars = 100 μm.3.4. TSLP transcriptional regulator AP1 is up-regulated and active in EGFR∆ep mice.