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. 2021 Nov 10;22(22):12187. doi: 10.3390/ijms222212187

Figure 2.

Figure 2

Cx26 (A), Cx32 (B) and Cx43 (C) gene expression in liver cancer cell lines and primary human hepatocytes (PHH). Cancer cell lines were grown to 100% confluence, while PHH were used in suspension when total RNA was extracted (n = 1, N = 3). Subsequently, real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) analysis was performed. Relative alterations compared to PHH were calculated according to the Pfaffl method in qbase+ (Biogazelle, Gent, Belgium). Data are expressed as mean ± standard deviation with * p ≤ 0.05 and **** p ≤ 0.0001 compared to the PHH control.