Table 1.

Overview of the liver cancer cell lines and primary human hepatocytes (PHH). Specifications for all cell types used in this study, including morphology, disease, age, gender, ethnicity, hepatitis B virus (HBV) infection of the donors and used cell culture medium (HCC, hepatocellular carcinoma; EMEM, Eagle’s minimum essential medium; FBS, fetal bovine serum; RPMI-1640, Roswell Park Memorial Institute 1640 Medium; CHRM, cryopreserved hepatocyte recovery medium; CHPM, cryopreserved hepatocyte plating medium).

Cell Line	Morphology	Disease	Age	Gender	Ethnicity	HBV	Medium
SK-HEP-1	Epithelial	Adenocarcinoma	52	Male	Caucasian	Not detected	EMEM + FBS
C3A	Epithelial	HCC	15	Male	Caucasian	Not detected	EMEM + FBS
SNU-449	Epithelial	Grade II-III/IV, HCC	52	Male	Asian	DNA detected	RPMI-1640 + heat-inactivated FBS
SNU-423	Epithelial	Grade III/IV, pleomorphic HCC	40	Male	Asian	DNA detected	RPMI-1640 + heat-inactivated FBS
SNU-387	Epithelial	Grade IV/V, pleomorphic HCC	41	Female	Asian	DNA detected	RPMI-1640 + FBS
SNU-475	Epithelial	Grade II-IV/V, HCC	43	Male	Asian	DNA detected	RPMI-1640 + heat-inactivated FBS
PLC/PRF/5	Epithelial	HCC	24	Male	African	Secreted HbsAg	EMEM + FBS
PHH	Epithelial	Liver metastasis of colorectal adenocarcinoma	74	Female	Caucasian	Not detected	CHRM + CHPM